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CoraLite® Plus 488-conjugated ASK1 Monoclonal antibody

ASK1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3A10

Cat no : CL488-67072

Print datasheet

Synonyms

ASK-1, MAP3K5, EC:2.7.11.25, ASK 1, Apoptosis signal-regulating kinase 1

验证数据展示

  • IF Staining of A431 using CL488-67072

    IF Staining of A431 using CL488-67072

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 ASK1 antibody (CL488-67072, Clone: 1C3A10 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-67072

    FC experiment of A431 using CL488-67072

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ASK1 (CL488-67072, Clone:1C3A10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67072-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67072 targets ASK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ASK1 fusion protein Ag5818 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 5
Synonyms ASK-1, MAP3K5, EC:2.7.11.25, ASK 1, Apoptosis signal-regulating kinase 1
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC054503
Gene Symbol ASK1
Gene ID (NCBI) 4217
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99683
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASK1 antibody CL488-67072Download protocol
FC protocol for CL Plus 488 ASK1 antibody CL488-67072Download protocol
Standard Protocols
Click here to view our Standard Protocols