验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 67072-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67072 targets ASK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ASK1 fusion protein Ag5818 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase kinase 5 |
| 别名 | ASK-1, MAP3K5, EC:2.7.11.25, ASK 1, Apoptosis signal-regulating kinase 1 |
| 计算分子量 | 155 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC054503 |
| 基因名称 | ASK1 |
| Gene ID (NCBI) | 4217 |
| RRID | AB_2934517 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99683 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ASK1 antibody CL488-67072 | Download protocol |
| FC protocol for CL Plus 488 ASK1 antibody CL488-67072 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |