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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATAT1 Polyclonal antibody, PBS Only

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 28828-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28828-1-PBS targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATAT1 fusion protein Ag28757 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 134
别名
计算分子量 47 kDa
观测分子量42-45 kDa
GenBank蛋白编号NM_001031722
基因名称 ATAT1
Gene ID (NCBI) 79969
RRIDAB_2881219
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SQI0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.