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  • Featured Product
  • KD/KO Validated

ATAT1 Polyclonal antibody, PBS Only

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 28828-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

产品信息

28828-1-PBS targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATAT1 fusion protein Ag28757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 134
Synonyms
Calculated Molecular Weight 47 kDa
Observed Molecular Weight42-45 kDa
GenBank Accession NumberNM_001031722
Gene Symbol ATAT1
Gene ID (NCBI) 79969
RRIDAB_2881219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SQI0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.