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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

    WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

ATF2 Polyclonal antibody, PBS Only

ATF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11908-1-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

11908-1-PBS targets ATF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATF2 fusion protein Ag2581 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activating transcription factor 2
Synonyms CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2
Calculated Molecular Weight 209 aa, 23 kDa
Observed Molecular Weight 60 kDa, 64 kDa
GenBank Accession NumberBC026175
Gene Symbol ATF2
Gene ID (NCBI) 1386
RRIDAB_10695892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15336
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).