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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

    WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

ATF2 Polyclonal antibody, PBS Only

ATF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11908-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11908-1-PBS targets ATF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ATF2 fusion protein Ag2581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activating transcription factor 2
别名 CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2
计算分子量 209 aa, 23 kDa
观测分子量 60 kDa, 64 kDa
GenBank蛋白编号BC026175
基因名称 ATF2
Gene ID (NCBI) 1386
RRIDAB_10695892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15336
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).