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  • Featured Product
  • KD/KO Validated

ATF4 Monoclonal antibody, PBS Only

ATF4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4C5

Cat no : 60035-1-PBS

Print datasheet

Synonyms

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

验证数据展示

  • WB analysis of HepG2 using 60035-1-Ig (same clone as 60035-1-PBS)

    WB analysis of HepG2 using 60035-1-Ig (same clone as 60035-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

  • WB analysis using 60035-1-Ig (same clone as 60035-1-PBS)

    WB analysis using 60035-1-Ig (same clone as 60035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60035-1-Ig (ATF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60035-1-Ig (same clone as 60035-1-PBS)

    FC experiment of HeLa using 60035-1-Ig (same clone as 60035-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

    IHC staining of human pancreas cancer using 60035-1-Ig (same clone as 60035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60035-1-Ig (ATF4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF4 antibody included
Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

产品信息

60035-1-PBS targets ATF4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATF4 fusion protein Ag1279 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC022088
Gene Symbol ATF4
Gene ID (NCBI) 468
RRIDAB_2058598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18848
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

 

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

 

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

 

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

 

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

 

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

 

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

 

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)