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ATF4 Recombinant antibody, PBS Only

ATF4 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N3

Cat no : 81798-1-PBS

Print datasheet

Synonyms

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

验证数据展示

  • WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    Non-treated HEK-293 and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

    Non-treated HeLa and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    IP result of anti-ATF4 (IP:81798-1-RR, 4ug; Detection:81798-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

    IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF4 antibody included
  • Affinity and Kinetic Characterization of 81798-1-RR

    Affinity and Kinetic Characterization of 81798-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81798-1-RR against Human ATF4 were performed. The affinity constant is 0.500 nM.

Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

产品信息

81798-1-PBS targets ATF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ATF4 fusion protein Ag1279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC022088
Gene Symbol ATF4
Gene ID (NCBI) 468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18848
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.