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验证数据展示

  • WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    Non-treated HEK-293 and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

    WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

    Non-treated HeLa and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

    IP result of anti-ATF4 (IP:81798-1-RR, 4ug; Detection:81798-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

    IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF4 antibody included
  • Affinity and Kinetic Characterization of 81798-1-RR

    Affinity and Kinetic Characterization of 81798-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81798-1-RR against Human ATF4 were performed. The affinity constant is 0.500 nM.

ATF4 Recombinant antibody, PBS Only

ATF4 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81798-1-PBS

产品说明书

CloneNo. 5N3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

5N3, cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

产品信息

81798-1-PBS targets ATF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 ATF4 fusion protein Ag1279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activating transcription factor 4 (tax-responsive enhancer element B67)
别名 5N3, cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67
计算分子量 39 kDa
观测分子量50 kDa
GenBank蛋白编号BC022088
基因名称 ATF4
Gene ID (NCBI) 468
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP18848
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.