ATF4 Recombinant antibody
ATF4 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
5N3
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, MG132 treated HEK-293 cells, MG132 treated HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81798-1-RR targets ATF4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Tested Reactivity | human |
Immunogen | ATF4 fusion protein Ag1279 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | activating transcription factor 4 (tax-responsive enhancer element B67) |
Synonyms | TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC022088 |
Gene Symbol | ATF4 |
Gene ID (NCBI) | 468 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P18848 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATF4 antibody 81798-1-RR | Download protocol |
IHC protocol for ATF4 antibody 81798-1-RR | Download protocol |
IP protocol for ATF4 antibody 81798-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |