• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATF4 Recombinant antibody

ATF4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230306B5

Cat no : 81798-2-RR

Print datasheet

Synonyms

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

验证数据展示

  • WB analysis using 81798-2-RR

    WB analysis using 81798-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81798-2-RR (ATF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81798-2-RR

    IF Staining of HeLa using 81798-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (81798-2-RR, Clone: 230306B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 81798-2-RR

    FC experiment of HEK-293 using 81798-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug ATF4 Recombinant antibody (81798-2-RR, Clone:230306B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81798-2-RR

    Affinity and Kinetic Characterization of 81798-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81798-2-RR against Human ATF4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells
Positive IF/ICC detected inTunicamycin treated HeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81798-2-RR targets ATF4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ATF4 fusion protein Ag1279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC022088
Gene Symbol ATF4
Gene ID (NCBI) 468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.

实验方案

Product Specific Protocols
WB protocol for ATF4 antibody 81798-2-RRDownload protocol
IF protocol for ATF4 antibody 81798-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols