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ATF6 Recombinant antibody, PBS Only

ATF6 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O8

Cat no : 82278-1-PBS

Print datasheet

Synonyms

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

验证数据展示

  • WB analysis using 82278-1-RR (same clone as 82278-1-PBS)

    WB analysis using 82278-1-RR (same clone as 82278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82278-1-RR (ATF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    WB analysis of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    WB result of ATF6 antibody (82278-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    IP experiment of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    IP result of anti-ATF6 (IP:82278-1-RR, 4ug; Detection:82278-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82278-1-RR

    Affinity and Kinetic Characterization of 82278-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82278-1-RR against Human ATF6 were performed. The affinity constant is 0.344 nM.

Planning an IHC experiment? We recommend our IHCeasy ATF6 Ready-To-Use IHC Kit. ATF6 primary antibody included.

产品信息

82278-1-PBS targets ATF6 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ATF6 fusion protein Ag21456 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activating transcription factor 6
Synonyms ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC014969
Gene Symbol ATF6
Gene ID (NCBI) 22926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18850
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).