验证数据展示
产品信息
82278-1-PBS targets ATF6 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, Indirect ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ATF6 fusion protein Ag21456 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | activating transcription factor 6 |
| 别名 | 2O8, activating transcription factor 6, ATF 6, ATF6 alpha, ATF6A |
| 计算分子量 | 75 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC014969 |
| 基因名称 | ATF6 |
| Gene ID (NCBI) | 22926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18850 |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).