验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82278-1-RR targets ATF6 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | ATF6 fusion protein Ag21456 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | activating transcription factor 6 |
| Synonyms | ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC014969 |
| Gene Symbol | ATF6 |
| Gene ID (NCBI) | 22926 |
| RRID | AB_3086474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18850 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATF6 antibody 82278-1-RR | Download protocol |
| IP protocol for ATF6 antibody 82278-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |