验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11262 targets ATG3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ATG3 fusion protein Ag1785 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATG3 autophagy related 3 homolog (S. cerevisiae) |
| 别名 | APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, DKFZp564M1178, FLJ22125, hApg3, PC3 96, Protein PC3 96 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36-40 kDa |
| GenBank蛋白编号 | BC024221 |
| 基因名称 | ATG3 |
| Gene ID (NCBI) | 64422 |
| RRID | AB_3672490 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NT62 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Autophagy is a lysosome-dependent conserved catabolic process in response to various stresses, such as oxidative stress and ischemia, for the degradation and recycling of aged or dysfunctional intracellular components and damaged organelles. A large group of autophagy-related genes (ATGs) have been identifed as essential drivers in diferent stages of autophagy. Autophagy-related 3 (ATG3), one of the autophagyrelated gene family members, serves as an E2-like enzyme contributing to the conjugation of microtubule-associated protein light chain 3 (LC3) to lipid phosphotidylethanolamine (PE). ATG3 plays a signifcant role in the regulation of autophagy, viability, and death.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATG3 antibody CL488-11262 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |