CoraLite®594-conjugated ATG4B Monoclonal antibody
ATG4B Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G5A7
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67727-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67727 targets ATG4B in IF applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | ATG4B fusion protein Ag30510 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ATG4 autophagy related 4 homolog B (S. cerevisiae) |
| Synonyms | APG4B, ATG4B, AUTL1, Autophagin 1, Cysteine protease ATG4B, hAPG4B, KIAA0943 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000719 |
| Gene Symbol | ATG4B |
| Gene ID (NCBI) | 23192 |
| RRID | AB_2920174 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4P1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ATG4B antibody CL594-67727 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |