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验证数据展示

  • WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

    WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

    IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00902-3

    Cytometric bead array standard curve of MP00902-3

    Cytometric bead array standard curve of MP00902-3, ATG4D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83951-1-PBS. Detection antibody: 83951-4-PBS. Standard: Ag10177. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83951-4-RR

    Affinity and Kinetic Characterization of 83951-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

ATG4D Recombinant antibody, PBS Only (Detector)

ATG4D Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83951-4-PBS

产品说明书

CloneNo. 241035G8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83951-4-PBS targets ATG4D as part of a matched antibody pair:

MP00902-3: 83951-1-PBS capture and 83951-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 ATG4D fusion protein Ag10177 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog D (S. cerevisiae)
别名 AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D
计算分子量 474 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC068992
基因名称 ATG4D
Gene ID (NCBI) 84971
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86TL0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.