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ATG4D Recombinant antibody, PBS Only (Detector)

ATG4D Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241035G8

Cat no : 83951-4-PBS

Print datasheet

Synonyms

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

验证数据展示

  • WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

    WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

    IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00902-3

    Cytometric bead array standard curve of MP00902-3

    Cytometric bead array standard curve of MP00902-3, ATG4D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83951-1-PBS. Detection antibody: 83951-4-PBS. Standard: Ag10177. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83951-4-RR

    Affinity and Kinetic Characterization of 83951-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

产品信息

83951-4-PBS targets ATG4D as part of a matched antibody pair:

MP00902-3: 83951-1-PBS capture and 83951-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen ATG4D fusion protein Ag10177 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATG4 autophagy related 4 homolog D (S. cerevisiae)
Synonyms AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC068992
Gene Symbol ATG4D
Gene ID (NCBI) 84971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86TL0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.