ATG4D Recombinant antibody

ATG4D Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241035G8

Cat no : 83951-4-RR

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Synonyms

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D



经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83951-4-RR targets ATG4D in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen ATG4D fusion protein Ag10177 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATG4 autophagy related 4 homolog D (S. cerevisiae)
Synonyms AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC068992
Gene Symbol ATG4D
Gene ID (NCBI) 84971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86TL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

实验方案

Product Specific Protocols
WB protocol for ATG4D antibody 83951-4-RRDownload protocol
IHC protocol for ATG4D antibody 83951-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols