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验证数据展示

  • WB analysis of A549 using 83951-4-RR

    WB analysis of A549 using 83951-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 83951-4-RR

    IHC staining of rat liver using 83951-4-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83951-4-RR

    Affinity and Kinetic Characterization of 83951-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

ATG4D Recombinant antibody

ATG4D Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83951-4-RR

产品说明书

CloneNo. 241035G8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83951-4-RR targets ATG4D in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 ATG4D fusion protein Ag10177 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog D (S. cerevisiae)
别名 AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D
计算分子量 474 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC068992
基因名称 ATG4D
Gene ID (NCBI) 84971
RRIDAB_3671530
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86TL0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

实验方案

Product Specific Protocols
WB protocol for ATG4D antibody 83951-4-RRDownload protocol
IHC protocol for ATG4D antibody 83951-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols