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验证数据展示

  • WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

    WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66744-1-Ig (same clone as 66744-1-PBS)

    WB analysis of HepG2 using 66744-1-Ig (same clone as 66744-1-PBS)

    WB result of ATG5 antibody (66744-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATG5 Monoclonal antibody, PBS Only

ATG5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66744-1-PBS

产品说明书

CloneNo. 2D3B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2D3B8, APG5, APG5 LIKE, APG5L, APG5-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66744-1-PBS targets ATG5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATG5 fusion protein Ag16685 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ATG5 autophagy related 5 homolog (S. cerevisiae)
别名 2D3B8, APG5, APG5 LIKE, APG5L, APG5-like
计算分子量 32 kDa
观测分子量 50-55 and 40-45 kDa
GenBank蛋白编号BC002699
基因名称 ATG5
Gene ID (NCBI) 9474
RRIDAB_2882092
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H1Y0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)