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验证数据展示

  • WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

    WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

    WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

    WB result of ATG5 antibody (66744-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HeLa cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATG5 Monoclonal antibody, PBS Only

ATG5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66744-1-PBS

Print datasheet

CloneNo. 2D3B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66744-1-PBS targets ATG5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATG5 fusion protein Ag16685 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Synonyms Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 50-55 and 40-45 kDa
GenBank Accession NumberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
RRIDAB_2882092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H1Y0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)