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ATG5 Monoclonal antibody
ATG5 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2D3B8
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66744-1-Ig targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | ATG5 fusion protein Ag16685 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
Synonyms | Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5 |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 50-55 and 40-45 kDa |
GenBank Accession Number | BC002699 |
Gene Symbol | ATG5 |
Gene ID (NCBI) | 9474 |
RRID | AB_2882092 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9H1Y0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATG5 antibody 66744-1-Ig | Download protocol |
IHC protocol for ATG5 antibody 66744-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy | ||
Dev Cell ATG5 attenuates inflammatory signaling in mouse embryonic stem cells to control differentiation
| ||
J Nanobiotechnology Cold exposure protects against medial arterial calcification development via autophagy | ||
J Nanobiotechnology Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.
| ||
Cancer Cell Int LncRNA LZTS1-AS1 induces proliferation, metastasis and inhibits autophagy of pancreatic cancer cells through the miR-532 /TWIST1 signaling pathway |