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CoraLite® Plus 750-conjugated ATG5 Recombinant antibody
ATG5 Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
5M5
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 81803-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81803 targets ATG5 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | ATG5 fusion protein Ag0214 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
| Synonyms | |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002699 |
| Gene Symbol | ATG5 |
| Gene ID (NCBI) | 9474 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H1Y0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 ATG5 antibody CL750-81803 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |