- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated ATG7 Monoclonal antibody
ATG7 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B5A10
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67341-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67341 targets ATG7 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | ATG7 fusion protein Ag29172 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
| Synonyms | APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 68-78 kDa |
| GenBank Accession Number | BC000091 |
| Gene Symbol | ATG7 |
| Gene ID (NCBI) | 10533 |
| RRID | AB_2919455 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95352 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATG7 antibody CL488-67341 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
