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ATG9A Monoclonal antibody, PBS Only

ATG9A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12E11

Cat no : 67096-1-PBS

Print datasheet

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

验证数据展示

  • WB analysis using 67096-1-Ig (same clone as 67096-1-PBS)

    WB analysis using 67096-1-Ig (same clone as 67096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67096-1-Ig (ATG9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67096-1-Ig (same clone as 67096-1-PBS)

    IF Staining of HepG2 using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67096-1-Ig (ATG9A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

产品信息

67096-1-PBS targets ATG9A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ATG9A fusion protein Ag24278 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Synonyms APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208
Calculated Molecular Weight 837 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC065534
Gene Symbol ATG9A
Gene ID (NCBI) 79065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z3C6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)