验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 2 publications below |
产品信息
67096-1-Ig targets ATG9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ATG9A fusion protein Ag24278 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATG9 autophagy related 9 homolog A (S. cerevisiae) |
| 别名 | APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208 |
| 计算分子量 | 837 aa, 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC065534 |
| 基因名称 | ATG9A |
| Gene ID (NCBI) | 79065 |
| RRID | AB_2882401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q7Z3C6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG9A antibody 67096-1-Ig | Download protocol |
| IHC protocol for ATG9A antibody 67096-1-Ig | Download protocol |
| IF protocol for ATG9A antibody 67096-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
NPJ Parkinsons Dis Regulators of proteostasis are translationally repressed in fibroblasts from patients with sporadic and LRRK2-G2019S Parkinson's disease | ||
Invest Ophthalmol Vis Sci HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation | ||
Drug Resist Updat NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1 | ||
