验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67096-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67096 targets ATG9A in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | ATG9A fusion protein Ag24278 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATG9 autophagy related 9 homolog A (S. cerevisiae) |
| 别名 | APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208 |
| 计算分子量 | 837 aa, 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC065534 |
| 基因名称 | ATG9A |
| Gene ID (NCBI) | 79065 |
| RRID | AB_2883397 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q7Z3C6 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATG9A antibody CL488-67096 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
