CoraLite® Plus 488-conjugated ATOH1 Polyclonal antibody
ATOH1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 21215-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-21215 targets ATOH1 in FC (Intra), IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | ATOH1 fusion protein Ag15615 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | atonal homolog 1 (Drosophila) |
| Synonyms | MATH 1, Helix-loop-helix protein hATH-1, HATH1, Class A basic helix-loop-helix protein 14, bHLHa14 |
| Calculated Molecular Weight | 354 aa, 38 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC069594 |
| Gene Symbol | ATOH1 |
| Gene ID (NCBI) | 474 |
| RRID | AB_2919180 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92858 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ATOH1 antibody CL488-21215 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
