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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83785-6-RR

    Affinity and Kinetic Characterization of 83785-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

ATOX1 Recombinant antibody, PBS Only

ATOX1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83785-6-PBS

产品说明书

CloneNo. 240679E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

ATX1, ATOX 1, Antioxidant 1, 240679E9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATOX1 Ready-To-Use IHC Kit. ATOX1 primary antibody included.

产品信息

83785-6-PBS targets ATOX1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ATOX1 fusion protein Ag18460 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATX1 antioxidant protein 1 homolog (yeast)
别名 ATX1, ATOX 1, Antioxidant 1, 240679E9
计算分子量 68 aa, 8 kDa
观测分子量 7 kDa
GenBank蛋白编号BC112248
基因名称 ATOX1
Gene ID (NCBI) 475
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00244
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).