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ATOX1 Recombinant antibody, PBS Only

ATOX1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240679E9

Cat no : 83785-6-PBS

Print datasheet

Synonyms

ATX1, ATOX 1, Antioxidant 1, 240679E9

验证数据展示

  • WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83785-6-RR

    Affinity and Kinetic Characterization of 83785-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

Planning an IHC experiment? We recommend our IHCeasy ATOX1 Ready-To-Use IHC Kit. ATOX1 primary antibody included.

产品信息

83785-6-PBS targets ATOX1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ATOX1 fusion protein Ag18460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATX1 antioxidant protein 1 homolog (yeast)
Synonyms ATX1, ATOX 1, Antioxidant 1, 240679E9
Calculated Molecular Weight 68 aa, 8 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC112248
Gene Symbol ATOX1
Gene ID (NCBI) 475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00244
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).