ATOX1 Recombinant antibody

ATOX1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83785-6-RR

Print datasheet

CloneNo. 240679E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

ATX1, ATOX 1, Antioxidant 1, 240679E9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, A549 cells, HEK-293 cells, NCI-H1299 cells, HepG2 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ATOX1 Ready-To-Use IHC Kit. ATOX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83785-6-RR targets ATOX1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ATOX1 fusion protein Ag18460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATX1 antioxidant protein 1 homolog (yeast)
Synonyms ATX1, ATOX 1, Antioxidant 1, 240679E9
Calculated Molecular Weight 68 aa, 8 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC112248
Gene Symbol ATOX1
Gene ID (NCBI) 475
RRIDAB_3671377
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).

实验方案

Product Specific Protocols
WB protocol for ATOX1 antibody 83785-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...