验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse heart tissue, rat heart tissue, fetal human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84707-5-RR targets ATP1B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ATP1B1 fusion protein Ag7279 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | ATPase, Na+/K+ transporting, beta 1 polypeptide |
别名 | Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1 |
计算分子量 | 35 kDa |
观测分子量 | 45-52 kDa |
GenBank蛋白编号 | BC000006 |
基因名称 | ATP1B1 |
Gene ID (NCBI) | 481 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P05026 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)
实验方案
Product Specific Protocols | |
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WB protocol for ATP1B1 antibody 84707-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |