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ATP1B1 Recombinant antibody

ATP1B1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242109D12

Cat no : 84707-5-RR

Print datasheet

Synonyms

Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1

验证数据展示

  • WB analysis using 84707-5-RR

    WB analysis using 84707-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84707-5-RR

    Affinity and Kinetic Characterization of 84707-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, rat heart tissue, fetal human brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84707-5-RR targets ATP1B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATP1B1 fusion protein Ag7279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Synonyms Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC000006
Gene Symbol ATP1B1
Gene ID (NCBI) 481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05026
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

实验方案

Product Specific Protocols
WB protocol for ATP1B1 antibody 84707-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols