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ATP1B1 Recombinant antibody, PBS Only

ATP1B1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242109D12

Cat no : 84707-5-PBS

Print datasheet

Synonyms

Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1

验证数据展示

  • WB analysis using 84707-5-RR (same clone as 84707-5-PBS)

    WB analysis using 84707-5-RR (same clone as 84707-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84707-5-RR

    Affinity and Kinetic Characterization of 84707-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

产品信息

84707-5-PBS targets ATP1B1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATP1B1 fusion protein Ag7279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Synonyms Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC000006
Gene Symbol ATP1B1
Gene ID (NCBI) 481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05026
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)