ATP5F1 Polyclonal antibody
ATP5F1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, IF, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse liver tissue |
Positive IHC detected in | human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15999-1-AP targets ATP5F1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | CoIP, IF, IHC, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | ATP5F1 fusion protein Ag8571 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 |
Synonyms | ATP5F1, ATPase subunit b, PIG47 |
Calculated Molecular Weight | 256 aa, 29 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC005366 |
Gene Symbol | ATP5F1 |
Gene ID (NCBI) | 515 |
RRID | AB_2258817 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P24539 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
IHC protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
IF protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation. | ||
Part Fibre Toxicol Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction. | ||
Free Radic Biol Med Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3. | ||
J Neurosci A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins. | ||
Cell Death Dis Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway. | ||
J Mol Biol COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria. |