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验证数据展示

  • IF Staining of HepG2 using 60434-1-Ig (same clone as 60434-1-PBS)

    IF Staining of HepG2 using 60434-1-Ig (same clone as 60434-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5L antibody (60434-1-Ig, Clone: 2D10F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60434-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50581-1

    Cytometric bead array standard curve of MP50581-1

    Cytometric bead array standard curve of MP50581-1, ATP5L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60434-1-PBS. Detection antibody: 60434-2-PBS. Standard:Ag9287. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50581-2

    Cytometric bead array standard curve of MP50581-2

    Cytometric bead array standard curve of MP50581-2, ATP5L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60434-1-PBS. Detection antibody: 60434-3-PBS. Standard:Ag9287. Range: 0.391-100 ng/mL.

ATP5L Monoclonal antibody, PBS Only (Capture)

ATP5L Monoclonal Antibody for IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60434-1-PBS

产品说明书

CloneNo. 2D10F9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, Cytometric bead array, Indirect ELISA

别名

ATPase subunit g, ATP5MG, ATP5JG, ATP synthase membrane subunit g, 2D10F9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60434-1-PBS targets ATP5L as part of a matched antibody pair:

MP50581-1: 60434-1-PBS capture and 60434-2-PBS detection (validated in Cytometric bead array)

MP50581-2: 60434-1-PBS capture and 60434-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ATP5L fusion protein Ag9287 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
别名 ATPase subunit g, ATP5MG, ATP5JG, ATP synthase membrane subunit g, 2D10F9
计算分子量 11 kDa
GenBank蛋白编号BC015128
基因名称 ATP5L
Gene ID (NCBI) 10632
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G Magarose purification
UNIPROT IDO75964
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5L gene encodes ATP synthase subunit g of the Fo complex.