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验证数据展示

  • IF Staining of HeLa using 83201-3-RR (same clone as 83201-3-PBS)

    IF Staining of HeLa using 83201-3-RR (same clone as 83201-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP6 antibody (83201-3-RR, Clone: 240041B6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83201-3-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83201-3-RR (same clone as 83201-3-PBS)

    FC experiment of U2OS using 83201-3-RR (same clone as 83201-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ATP6 Recombinant antibody (83201-3-RR, Clone:240041B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83201-3-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 83201-3-RR (same clone as 83201-3-PBS)

    IF Staining of rat brain using 83201-3-RR (same clone as 83201-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ATP6 antibody (83201-3-RR, Clone: 240041B6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83201-3-PBS in a different storage buffer formulation.

ATP6 Recombinant antibody, PBS Only

ATP6 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 83201-3-PBS

产品说明书

CloneNo. 240041B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83201-3-PBS targets ATP6 in IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP synthase 6; ATPase subunit 6
别名 MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6
计算分子量 25 kDa
GenBank蛋白编号J01415
基因名称 ATP6
Gene ID (NCBI) 4508
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00846
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.