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验证数据展示

  • IF Staining of HeLa using 83201-3-RR

    IF Staining of HeLa using 83201-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP6 antibody (83201-3-RR, Clone: 240041B6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83201-3-RR

    FC experiment of U2OS using 83201-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ATP6 Recombinant antibody (83201-3-RR, Clone:240041B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of rat brain using 83201-3-RR

    IF Staining of rat brain using 83201-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ATP6 antibody (83201-3-RR, Clone: 240041B6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6 Recombinant antibody

ATP6 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83201-3-RR

产品说明书

CloneNo. 240041B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P, FC (Intra), ELISA

别名

MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:175-1:700
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83201-3-RR targets ATP6 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP synthase 6; ATPase subunit 6
别名 MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6
计算分子量 25 kDa
GenBank蛋白编号J01415
基因名称 ATP6
Gene ID (NCBI) 4508
RRIDAB_3670889
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00846
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.

实验方案

Product Specific Protocols
IF protocol for ATP6 antibody 83201-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols