验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15305-1-AP targets ATP6AP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ATP6AP1 fusion protein Ag7859 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+ transporting, lysosomal accessory protein 1 |
| 别名 | 16A, Ac45, ATP6AP1, ATP6IP1, ATP6S1, CF2, Protein XAP 3, V ATPase Ac45 subunit, V ATPase S1 accessory protein, V ATPase subunit S1, VATPS1, XAP 3, XAP3 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 46-52 kDa, 61-64 kDa |
| GenBank蛋白编号 | BC000724 |
| 基因名称 | ATP6AP1 |
| Gene ID (NCBI) | 537 |
| RRID | AB_10804756 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15904 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6AP1 antibody 15305-1-AP | Download protocol |
| IHC protocol for ATP6AP1 antibody 15305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteomes Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells. | ||
Sci Rep ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification | ||
Cancer Cell Int Lysosomal gene ATP6AP1 promotes doxorubicin resistance via up-regulating autophagic flux in breast cancer |
