• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATP6AP1 Polyclonal antibody

ATP6AP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15305-1-AP

Print datasheet

Synonyms

16A, Ac45, ATP6AP1, ATP6IP1, ATP6S1, CF2, Protein XAP 3, V ATPase Ac45 subunit, V ATPase S1 accessory protein, V ATPase subunit S1, VATPS1, XAP 3, XAP3

验证数据展示

  • WB analysis of mouse kidney using 15305-1-AP

    WB analysis of mouse kidney using 15305-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15305-1-AP

    IHC staining of human kidney using 15305-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 15305-1-AP

    IHC staining of human kidney using 15305-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15305-1-AP targets ATP6AP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ATP6AP1 fusion protein Ag7859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal accessory protein 1
Synonyms 16A, Ac45, ATP6AP1, ATP6IP1, ATP6S1, CF2, Protein XAP 3, V ATPase Ac45 subunit, V ATPase S1 accessory protein, V ATPase subunit S1, VATPS1, XAP 3, XAP3
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 46-52 kDa, 61-64 kDa
GenBank Accession NumberBC000724
Gene Symbol ATP6AP1
Gene ID (NCBI) 537
RRIDAB_10804756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15904
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6AP1 antibody 15305-1-APDownload protocol
IHC protocol for ATP6AP1 antibody 15305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Oncotarget

PMID: 28700346

Authors - Enrica Balza
View Article
humanWB

Proteomes

Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells.

Authors - Ang Luo
View Article
humanIHC

Sci Rep

ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification

Authors - Shijie Zhang
View Article