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ATP6V1B1 Recombinant antibody, PBS Only

ATP6V1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240955B9

Cat no : 83953-5-PBS

Print datasheet

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

验证数据展示

  • WB analysis using 83953-5-RR (same clone as 83953-5-PBS)

    WB analysis using 83953-5-RR (same clone as 83953-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

    IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

产品信息

83953-5-PBS targets ATP6V1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATP6V1B1 fusion protein Ag6332 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Synonyms V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15313
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.