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ATP6V1B1 Recombinant antibody

ATP6V1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240955B9

Cat no : 83953-5-RR

Print datasheet

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

验证数据展示

  • WB analysis using 83953-5-RR

    WB analysis using 83953-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 83953-5-RR

    IHC staining of rat kidney using 83953-5-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83953-5-RR targets ATP6V1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATP6V1B1 fusion protein Ag6332 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Synonyms V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

实验方案

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 83953-5-RRDownload protocol
IHC protocol for ATP6V1B1 antibody 83953-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols