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验证数据展示

  • WB analysis using 83953-5-RR

    WB analysis using 83953-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 83953-5-RR

    IHC staining of rat kidney using 83953-5-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V1B1 Recombinant antibody

ATP6V1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83953-5-RR

产品说明书

CloneNo. 240955B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83953-5-RR targets ATP6V1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATP6V1B1 fusion protein Ag6332 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
别名 V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1
计算分子量 57 kDa
观测分子量 56 kDa
GenBank蛋白编号BC063411
基因名称 ATP6V1B1
Gene ID (NCBI) 525
RRIDAB_3671532
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP15313
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

实验方案

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 83953-5-RRDownload protocol
IHC protocol for ATP6V1B1 antibody 83953-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols