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验证数据展示

  • WB analysis of mouse brain using 13235-1-AP

    WB analysis of mouse brain using 13235-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13235-1-AP (ATP6V1E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13235-1-AP

    IP experiment of mouse brain using 13235-1-AP

    IP result of anti-ATP6V1E2 (IP:13235-1-AP, 4ug; Detection:13235-1-AP 1:1000) with mouse brain tissue lysate 3000 ug.

ATP6V1E2 Polyclonal antibody

ATP6V1E2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13235-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

ATP6E1, ATP6EL2, ATP6V1E2, ATP6V1EL2, V ATPase subunit E 2, VMA4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13235-1-AP targets ATP6V1E2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATP6V1E2 fusion protein Ag4088 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
别名 ATP6E1, ATP6EL2, ATP6V1E2, ATP6V1EL2, V ATPase subunit E 2, VMA4
计算分子量 226 aa, 26 kDa
观测分子量 31-33 kDa
GenBank蛋白编号BC034808
基因名称 ATP6V1E2
Gene ID (NCBI) 90423
RRIDAB_2062675
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96A05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
IP protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols