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验证数据展示

  • WB analysis using 68351-1-Ig

    WB analysis using 68351-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68351-1-Ig

    IF Staining of HepG2 using 68351-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse heart using 68351-1-Ig

    IHC staining of mouse heart using 68351-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATPAF2 Monoclonal antibody

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68351-1-Ig

产品说明书

CloneNo. 1H9A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68351-1-Ig targets ATPAF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 ATPAF2 fusion protein Ag29065 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase mitochondrial F1 complex assembly factor 2
别名 ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663
计算分子量 289 aa, 33 kDa
观测分子量~30 kDa
GenBank蛋白编号BC032126
基因名称 ATPAF2
Gene ID (NCBI) 91647
RRIDAB_3085074
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N5M1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)

实验方案

Product Specific Protocols
WB protocol for ATPAF2 antibody 68351-1-IgDownload protocol
IHC protocol for ATPAF2 antibody 68351-1-IgDownload protocol
IF protocol for ATPAF2 antibody 68351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols