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ATPAF2 Monoclonal antibody, PBS Only (Capture)

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9A9

Cat no : 68351-1-PBS

Print datasheet

Synonyms

ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9

验证数据展示

  • WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

    WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

    IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

    IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50625-1

    Cytometric bead array standard curve of MP50625-1

    Cytometric bead array standard curve of MP50625-1, ATPAF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68351-1-PBS. Detection antibody: 68351-2-PBS. Standard:Ag29065. Range: 0.098-6.25 ng/mL.

产品信息

68351-1-PBS targets ATPAF2 as part of a matched antibody pair:

MP50625-1: 68351-1-PBS capture and 68351-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATPAF2 fusion protein Ag29065 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase mitochondrial F1 complex assembly factor 2
Synonyms ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight~30 kDa
GenBank Accession NumberBC032126
Gene Symbol ATPAF2
Gene ID (NCBI) 91647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5M1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)