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验证数据展示

  • WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

    WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

    IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

    IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50625-1

    Cytometric bead array standard curve of MP50625-1

    Cytometric bead array standard curve of MP50625-1, ATPAF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68351-1-PBS. Detection antibody: 68351-2-PBS. Standard:Ag29065. Range: 0.098-6.25 ng/mL.

ATPAF2 Monoclonal antibody, PBS Only (Capture)

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68351-1-PBS

产品说明书

CloneNo. 1H9A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68351-1-PBS targets ATPAF2 as part of a matched antibody pair:

MP50625-1: 68351-1-PBS capture and 68351-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATPAF2 fusion protein Ag29065 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase mitochondrial F1 complex assembly factor 2
别名 ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9
计算分子量 289 aa, 33 kDa
观测分子量~30 kDa
GenBank蛋白编号BC032126
基因名称 ATPAF2
Gene ID (NCBI) 91647
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N5M1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)