CoraLite® Plus 488-conjugated ATPB Monoclonal antibody
ATPB Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse , Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A11B9
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66600-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66600 targets ATPB in IF/ICC applications and shows reactivity with Human, Mouse , Rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse , Rat |
Immunogen | ATPB fusion protein Ag11177 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
Synonyms | ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrion Marker |
Calculated Molecular Weight | 529 aa, 57 kDa |
Observed Molecular Weight | 45-57 kDa |
GenBank Accession Number | BC016512 |
Gene Symbol | ATPB |
Gene ID (NCBI) | 506 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P06576 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ATPB antibody CL488-66600 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |