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ATX3,ATXN3 Monoclonal antibody, PBS Only

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat no : 67057-1-PBS

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Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

验证数据展示

  • WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

    WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

产品信息

67057-1-PBS targets ATX3,ATXN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen ATX3,ATXN3 fusion protein Ag6627 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ataxin 3
Synonyms AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC033711
Gene Symbol ATXN3
Gene ID (NCBI) 4287
RRIDAB_2882368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.