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ATX3,ATXN3 Monoclonal antibody, PBS Only

ATX3,ATXN3 Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat No : 67057-1-PBS

Print datasheet

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

验证数据展示

  • WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

    WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

产品信息

67057-1-PBS targets ATX3,ATXN3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human, mouse, pig
Immunogen ATX3,ATXN3 fusion protein Ag6627 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ataxin 3
Synonyms AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC033711
Gene Symbol ATXN3
Gene ID (NCBI) 4287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.