ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67057-1-Ig

产品说明书

CloneNo. 1F7E10

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67057-1-Ig targets ATX3,ATXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse, pig
文献引用反应性human, mouse
免疫原 ATX3,ATXN3 fusion protein Ag6627 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ataxin 3
别名 AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3
计算分子量 370 aa, 43 kDa
观测分子量 41-45 kDa
GenBank蛋白编号BC033711
基因名称 ATXN3
Gene ID (NCBI) 4287
RRIDAB_2882368
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

实验方案

Product Specific Protocols
WB protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
IHC protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Clin Invest

CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion

Authors - Shengnan Wang
humanWB

J Biol Chem

ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma

Authors - Yang Cheng
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