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验证数据展示

  • IF Staining of A549 using CL488-67057

    IF Staining of A549 using CL488-67057

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 ATX3,ATXN3 antibody (CL488-67057, Clone: 1F7E10 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of MCF-7 using CL488-67057

    FC experiment of MCF-7 using CL488-67057

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATX3,ATXN3 (CL488-67057, Clone:1F7E10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67057

产品说明书

CloneNo. 1F7E10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

IF/ICC, FC (Intra)

别名

ATXN3, ATX3, Ataxin-3, ataxin 3, AT3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67057-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67057 targets ATX3,ATXN3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, pig
免疫原 ATX3,ATXN3 fusion protein Ag6627 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ataxin 3
别名 ATXN3, ATX3, Ataxin-3, ataxin 3, AT3
计算分子量 370 aa, 43 kDa
观测分子量 41-45 kDa
GenBank蛋白编号BC033711
基因名称 ATXN3
Gene ID (NCBI) 4287
RRIDAB_3084303
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54252
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATX3,ATXN3 antibody CL488-67057Download protocol
Standard Protocols
Click here to view our Standard Protocols