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CoraLite® Plus 488-conjugated ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F7E10

Cat no : CL488-67057

Print datasheet

Synonyms

ATXN3, ATX3, Ataxin-3, ataxin 3, AT3

验证数据展示

  • IF Staining of A549 using CL488-67057

    IF Staining of A549 using CL488-67057

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 ATX3,ATXN3 antibody (CL488-67057, Clone: 1F7E10 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of MCF-7 using CL488-67057

    FC experiment of MCF-7 using CL488-67057

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATX3,ATXN3 (CL488-67057, Clone:1F7E10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67057-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67057 targets ATX3,ATXN3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, pig
Immunogen ATX3,ATXN3 fusion protein Ag6627 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ataxin 3
Synonyms ATXN3, ATX3, Ataxin-3, ataxin 3, AT3
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC033711
Gene Symbol ATXN3
Gene ID (NCBI) 4287
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54252
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATX3,ATXN3 antibody CL488-67057Download protocol
Standard Protocols
Click here to view our Standard Protocols