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AXIN1 Monoclonal antibody

AXIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C4E8

Cat no : 68093-1-Ig

Print datasheet

Synonyms

hAxin, Axis inhibition protein 1, Axin-1, AXIN 1, AXIN

验证数据展示

  • WB analysis using 68093-1-Ig

    WB analysis using 68093-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IHC staining of mouse stomach using 68093-1-Ig

    IHC staining of mouse stomach using 68093-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AXIN1 antibody included
  • IHC staining of mouse stomach using 68093-1-Ig

    IHC staining of mouse stomach using 68093-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AXIN1 antibody included
  • IF Staining of A431 using 68093-1-Ig

    IF Staining of A431 using 68093-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AXIN1 antibody (68093-1-Ig, Clone: 1C4E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of A431 using 68093-1-Ig

    FC experiment of A431 using 68093-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human AXIN1 (68093-1-Ig, Clone:1C4E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, HSC-T6 cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy AXIN1 Ready-To-Use IHC Kit. AXIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

68093-1-Ig targets AXIN1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AXIN1 fusion protein Ag10079 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name axin 1
Synonyms hAxin, Axis inhibition protein 1, Axin-1, AXIN 1, AXIN
Calculated Molecular Weight826aa,92 kDa; 862aa,95 kDa
Observed Molecular Weight110-120 kDa
GenBank Accession NumberBC044648
Gene Symbol AXIN1
Gene ID (NCBI) 8312
RRIDAB_2918830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC.

实验方案

Product Specific Protocols
WB protocol for AXIN1 antibody 68093-1-IgDownload protocol
IHC protocol for AXIN1 antibody 68093-1-IgDownload protocol
IF protocol for AXIN1 antibody 68093-1-IgDownload protocol
FC protocol for AXIN1 antibody 68093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Preliminary investigation on the mechanism of baicalein regulating the effects of Nischarin on invasion and apoptosis of human breast cancer cells MCF-7 through Wnt3α/β-catenin pathway

Authors - Gaojian He
View Article
WB

Int J Biol Macromol

Xylooligosaccharides alleviate the carbohydrate-enriched diet-induced intestinal barrier dysfunction in carp Megalobrama amblycephala by promoting intestinal development, immunity and gut microbiota

Authors - Wei-Liang Chen
View Article