验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68093 targets AXIN1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | AXIN1 fusion protein Ag10079 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | axin 1 |
| Synonyms | AXIN1, Axin-1, Axis inhibition protein 1, hAxin, 1C4E8, AXIN, AXIN 1 |
| Calculated Molecular Weight | 826aa,92 kDa; 862aa,95 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC044648 |
| Gene Symbol | AXIN1 |
| Gene ID (NCBI) | 8312 |
| RRID | AB_3084397 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15169 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.