验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68093 targets AXIN1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | AXIN1 fusion protein Ag10079 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | axin 1 |
| 别名 | AXIN1, Axin-1, Axis inhibition protein 1, hAxin, 1C4E8, AXIN, AXIN 1 |
| 计算分子量 | 826aa,92 kDa; 862aa,95 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC044648 |
| 基因名称 | AXIN1 |
| Gene ID (NCBI) | 8312 |
| RRID | AB_3084397 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O15169 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.