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验证数据展示

  • WB analysis using 68108-1-Ig

    WB analysis using 68108-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68108-1-Ig (AXL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

AXL Monoclonal antibody

AXL Monoclonal Antibody for WB, ELISA
Cat No. 68108-1-Ig

Print datasheet

CloneNo. 3D12F11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

UFO, Tyrosine-protein kinase receptor UFO, EC:2.7.10.1, 3D12F11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, A549 cells, U2OS cells, A431 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68108-1-Ig targets AXL in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen AXL fusion protein Ag14401 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name AXL receptor tyrosine kinase
Synonyms UFO, Tyrosine-protein kinase receptor UFO, EC:2.7.10.1, 3D12F11
Calculated Molecular Weight 894 aa, 98 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC032229
Gene Symbol AXL
Gene ID (NCBI) 558
RRIDAB_2923637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

实验方案

Product Specific Protocols
WB protocol for AXL antibody 68108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols