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验证数据展示

  • IF Staining of HeLa using CL488-68326

    IF Staining of HeLa using CL488-68326

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 AZI2 antibody (CL488-68326, Clone: 2F1G5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68326

    FC experiment of HeLa using CL488-68326

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AZI2 Monoclonal antibody (CL488-68326, Clone:2F1G5)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated AZI2 Monoclonal antibody

AZI2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68326

产品说明书

CloneNo. 2F1G5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

AZI2/NAP1, Nak-associated protein 1, Nak associated protein 1, 5-azacytidine-induced protein 2, 5 azacytidine induced 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68326-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68326 targets AZI2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 AZI2 fusion protein Ag6755 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5-azacytidine induced 2
别名 AZI2/NAP1, Nak-associated protein 1, Nak associated protein 1, 5-azacytidine-induced protein 2, 5 azacytidine induced 2
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC001139
基因名称 AZI2
Gene ID (NCBI) 64343
RRIDAB_3084474
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H6S1
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. NAP1 is a kind of adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. (PMID: 21931631)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AZI2 antibody CL488-68326Download protocol
FC protocol for CL Plus 488 AZI2 antibody CL488-68326Download protocol
Standard Protocols
Click here to view our Standard Protocols