• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AbFlex BMI-1 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, WB

Cat No : 91195,91196 91195

Synonyms

BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, stem cells

验证数据展示

  • Western blot of AbFlex BMI-1 antibody (rAb). 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with AbFlex BMI-1 antibody.

    Western blot of AbFlex BMI-1 antibody (rAb). 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with AbFlex BMI-1 antibody.

  • AbFlex BMI-1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of mouse embryonic fibroblast (MEF) chromatin and 4 ug of BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.9 million sequence tags were mapped to identify BMI-1 binding sites on chromosome 2.

    AbFlex BMI-1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of mouse embryonic fibroblast (MEF) chromatin and 4 ug of BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.9 million sequence tags were mapped to identify BMI-1 binding sites on chromosome 2.

产品信息

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB*: 0.5-2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39993.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against full length human BMI1 [BMI1 polycomb ring finger oncogene].
Full Name AbFlex BMI-1 antibody (rAb)
Synonyms BMI-1, BMI 1, BMI1, Polycomb, PRC1 complex, silencing, E3 ubiquitin protein ligase, RNF2, RING2, resulting in the ubiquitylation, histone H2A at lysine 119, H2AK119Ub, oncogene, lymphoma, metastatic melanoma, hematopoietic stem cell self renewal, stem cell, stem cells
Molecular weight
GenBank accession numberNP_005171
RRIDAB_2793798
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.07% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.