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AbFlex Histone H3K9ac antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, ELISA, ICC, IF, WB

Cat No : 91103,91104 91103

Synonyms

Histone H3, Histone acetylation, transcriptional activation, chromatin, nucleosome, antibody, antibodies, polyclonal, H3 AcK9, H3K9Ac, chromatin immunoprecipitation, chip, recombinant, rAb, rec Ab, AbFlex

验证数据展示

  • AbFlex Histone H3K9ac antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from PC9 cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 1.

    AbFlex Histone H3K9ac antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from PC9 cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 1.

  • AbFlex Histone H3K9ac antibody (rAb) tested by immunofluorescence. HeLa cell stained with 2ug /mL of AbFlex Histone H3K9ac antibody (rAb) followed by anti-mouse-IgG-488.

    AbFlex Histone H3K9ac antibody (rAb) tested by immunofluorescence. HeLa cell stained with 2ug /mL of AbFlex Histone H3K9ac antibody (rAb) followed by anti-mouse-IgG-488.

  • AbFlex Histone H3K9ac antibody (rAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with AbFlex Histone H3K9ac antibody (2 ug/ml dilution). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate.

    AbFlex Histone H3K9ac antibody (rAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with AbFlex Histone H3K9ac antibody (2 ug/ml dilution). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate.

  • AbFlex Histone H3K9ac antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H3K9ac antibody (rAb) antibody for acetyl-lysine 9 H3. Peptides corresponding to regions around major sites of histone H3 acetylation or other acetyl-lysine peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexHistone H3K9ac antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

    AbFlex Histone H3K9ac antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H3K9ac antibody (rAb) antibody for acetyl-lysine 9 H3. Peptides corresponding to regions around major sites of histone H3 acetylation or other acetyl-lysine peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexHistone H3K9ac antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

产品信息

Tested Applications ChIP, ChIP-Seq, ELISA, ICC, IF, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug each ICC/IF: 1 - 2 ug/ml WB*: 0.2 - 2 ug/ml Bead-based ELISA: 1.5 - 40 ug/ml CUT&Tag: 1 ug per 50 ul reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental antibody clone, see Catalog No. 61251.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
ModificationAcetylated
Immunogen This antibody was raised against a peptide containing acetyl-lysine 9 of human Histone H3.
Full Name AbFlex Histone H3K9ac antibody (rAb)
Synonyms Histone H3, Histone acetylation, transcriptional activation, chromatin, nucleosome, antibody, antibodies, polyclonal, H3 AcK9, H3K9Ac, chromatin immunoprecipitation, chip, recombinant, rAb, rec Ab, AbFlex
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793775
Purification Method Ni-NTA
Buffer Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K9ac antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions.Acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes.