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Acetyl-Histone H2B (Lys120) Recombinant antibody, PBS Only

Acetyl-Histone H2B (Lys120) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241556E7

Cat no : 84551-1-PBS

Print datasheet

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

验证数据展示

  • Dot Blot experiment of peptide using 84551-1-RR (same clone as 84551-1-PBS)

    Dot Blot experiment of peptide using 84551-1-RR (same clone as 84551-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

    IF Staining of HeLa using 84551-1-RR (same clone as 84551-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 84551-1-RR (same clone as 84551-1-PBS)

    WB analysis of NIH/3T3 using 84551-1-RR (same clone as 84551-1-PBS)

    Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84551-1-PBS in a different storage buffer formulation.

产品信息

84551-1-PBS targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Dot Blot, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
Calculated Molecular Weight 14 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).