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Acetyl-Histone H2B (Lys120) Recombinant antibody

Acetyl-Histone H2B (Lys120) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241556E7

Cat no : 84551-1-RR

Print datasheet

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

验证数据展示

  • Dot Blot experiment of peptide using 84551-1-RR

    Dot Blot experiment of peptide using 84551-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • IF Staining of HeLa using 84551-1-RR

    IF Staining of HeLa using 84551-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of NIH/3T3 using 84551-1-RR

    WB analysis of NIH/3T3 using 84551-1-RR

    Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inTrichostatin A treated HeLa cells
Positive Dot Blot detected inpeptide

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
Calculated Molecular Weight 14 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols