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验证数据展示

  • Dot Blot experiment of peptide using 84551-1-RR

    Dot Blot experiment of peptide using 84551-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • IF Staining of HeLa using 84551-1-RR

    IF Staining of HeLa using 84551-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of NIH/3T3 using 84551-1-RR

    WB analysis of NIH/3T3 using 84551-1-RR

    Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Acetyl-Histone H2B (Lys120) Recombinant antibody

Acetyl-Histone H2B (Lys120) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 84551-1-RR

产品说明书

CloneNo. 241556E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Dot Blot, ELISA

别名

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inTrichostatin A treated HeLa cells
Positive Dot Blot detected inpeptide

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
计算分子量 14 kDa
观测分子量25 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3672059
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols