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验证数据展示

  • Dot Blot experiment of peptide using 84446-2-RR

    Dot Blot experiment of peptide using 84446-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis of HSC-T6 using 84446-2-RR

    WB analysis of HSC-T6 using 84446-2-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84446-2-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 84446-2-RR

    WB analysis of NIH/3T3 using 84446-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-2-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84446-2-RR

    IF Staining of HeLa using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of NIH/3T3 using 84446-2-RR

    IF Staining of NIH/3T3 using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of C6 using 84446-2-RR

    IF Staining of C6 using 84446-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated C6 cells using Acetyl-Histone H2B (Lys16) antibody (84446-2-RR, Clone: 241194C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Acetyl-Histone H2B (Lys16) Recombinant antibody

Acetyl-Histone H2B (Lys16) Uni-rAbTM Recombinant Antibody for IF/ICC, Dot Blot, ELISA
Cat No. 84446-2-RR

产品说明书

CloneNo. 241194C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, Dot Blot, ELISA

别名

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, Trichostatin A treated NIH/3T3 cells, Trichostatin A treated C6 cells
Positive Dot Blot detected inpeptide

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84446-2-RR targets Acetyl-Histone H2B (Lys16) in IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
计算分子量 14 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3671972
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys16) antibody 84446-2-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys16) antibody 84446-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols