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Acetyl-Histone H2B (Lys20) Recombinant antibody

Acetyl-Histone H2B (Lys20) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241204H8

Cat no : 84549-1-RR

Print datasheet

Synonyms

Acetyl Histone H2B, Ac Histone H2B-Lys20, Ac Histone H2B Lys20, Ac Histone H2B (Lys20), Ac Histone H2B

验证数据展示

  • Dot Blot experiment of peptide using 84549-1-RR

    Dot Blot experiment of peptide using 84549-1-RR

    Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis of NIH/3T3 using 84549-1-RR

    WB analysis of NIH/3T3 using 84549-1-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of HeLa using 84549-1-RR

    IF Staining of HeLa using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84549-1-RR targets Acetyl-Histone H2B (Lys20) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms Acetyl Histone H2B, Ac Histone H2B-Lys20, Ac Histone H2B Lys20, Ac Histone H2B (Lys20), Ac Histone H2B
Calculated Molecular Weight 14 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols