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验证数据展示

  • Dot Blot experiment of peptide using 82832-1-RR

    Dot Blot experiment of peptide using 82832-1-RR

    Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS).

  • WB analysis of NIH/3T3 using 82832-1-RR

    WB analysis of NIH/3T3 using 82832-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 82832-1-RR

    WB analysis of HSC-T6 using 82832-1-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 82832-1-RR

    WB analysis of NIH/3T3 using 82832-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Acetyl-Histone H3 (Lys18) Recombinant antibody

Acetyl-Histone H3 (Lys18) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 82832-1-RR
CloneNo. 2E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, ELISA

Synonyms

Acetyl Histone H3, Ac Histone H3-Lys18, Ac Histone H3 Lys18, Ac Histone H3 (Lys18), Ac Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells, HSC-T6 cells, NIH/3T3 cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82832-1-RR targets Acetyl-Histone H3 (Lys18) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms Acetyl Histone H3, Ac Histone H3-Lys18, Ac Histone H3 Lys18, Ac Histone H3 (Lys18), Ac Histone H3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3670568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys18) antibody 82832-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols